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human muc1 antigen  (Sino Biological)


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    Structured Review

    Sino Biological human muc1 antigen
    Human Muc1 Antigen, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+muc1+antigen/Human+Mucin-1+%2F+MUC-1+Protein/pm35021484-27-9-15
    Average 90 stars, based on 2 article reviews
    human muc1 antigen - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Combined Detection of CA19-9 and MUC1 Using a Colorimetric Immunosensor Based on Magnetic Gold Nanorods for Ultrasensitive Risk Assessment of Pancreatic Cancer.
    Article Snippet: We herein report a facile approach for developing an enzyme-free colorimetric immunosensor based on a magnetic iron oxide (IO)-coated gold nanorod (MGNR) nanocomposite with high electron transfer ability to accelerate the color bleaching reaction of methyl orange (MO) in the presence of NaBH4 for ultrasensitive detection of cancer antigens.. In the case of MO, the reaction rate of MGNRs showed approximately 45.6-fold and 1520.8-fold higher than that of Cys-GNRs and NaBH4, respectively.. The proposed colorimetric immunosensor was demonstrated to enable simple, cost-effective, sensitive, and specific carbohydrate antigen 19−9 (CA19−9) and mucin 1 (MUC1) detection for risk evaluation of pancreatic cancer (PC) with a small volume of serum sample without the use of any enhancing solutions or enzymes.



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    RSVA infection and mucin production in A549 spheroids. A549 Spheroids were inoculated with 1 MOI of RSVA 2001/2-20 and incubated for 3 (3A) and 7 (3B ) days. At the assigned time-points, spheroids were immunostained with anti-RSV F antibody (green), anti-MUC1 antibody (red), and counterstained with the nuclear stain DAPI (blue) and visualized by confocal microscopy at 10× magnification (n = 3). Syncytial formation in RSV infected spheroids at day 3 post-infection was also shown in insets at 40× magnification. (3C) Mucin accumulation and (3D) RSV-F dissemination were measured by the Zeiss LSM710 laser scanning confocal microscope. Mucin intensity fold change was evaluated between infected spheroids and their time matching controls (n = 3). RSV-F intensity fold change was evaluated between day 3 and day 7 infected spheroids (n = 3). Error bars represent ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Infection and Public Health

    Article Title: A three-dimensional A549 cell culture model to study respiratory syncytial virus infections

    doi: 10.1016/j.jiph.2020.03.011

    Figure Lengend Snippet: RSVA infection and mucin production in A549 spheroids. A549 Spheroids were inoculated with 1 MOI of RSVA 2001/2-20 and incubated for 3 (3A) and 7 (3B ) days. At the assigned time-points, spheroids were immunostained with anti-RSV F antibody (green), anti-MUC1 antibody (red), and counterstained with the nuclear stain DAPI (blue) and visualized by confocal microscopy at 10× magnification (n = 3). Syncytial formation in RSV infected spheroids at day 3 post-infection was also shown in insets at 40× magnification. (3C) Mucin accumulation and (3D) RSV-F dissemination were measured by the Zeiss LSM710 laser scanning confocal microscope. Mucin intensity fold change was evaluated between infected spheroids and their time matching controls (n = 3). RSV-F intensity fold change was evaluated between day 3 and day 7 infected spheroids (n = 3). Error bars represent ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: After 3 and 7 days post-inoculation, spheroids were fixed with 4% paraformaldehyde (416785000 ACROS) for 15 min and permeabilized with 0.2% Triton X-100 (T9284-100 ml Sigma) in PBS for 20 min. Spheroids were blocked with blocking solution [1% BSA (A3912-100 g Sigma) and 0.1% Triton X-100 in PBS] for 1 h followed by overnight incubation with a mixture of mouse anti-RSV fusion protein antibody (MAB8599-Merck Millipore) at 1:200 and rabbit anti-human MUC1 antibody (12123-T24 Sino Biological) at 1:2500 in blocking buffer.

    Techniques: Infection, Incubation, Staining, Confocal Microscopy, Microscopy